文章基本信息

标题：Improvement of Fructanohydrolase Production in Aspergillus niger SL-09 by Sucrose Ester
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作者：Zhang, Wei-Guo ; Ge, Xiang-Yang
期刊名称：Food Technology and Biotechnology
印刷版ISSN：1330-9862
电子版ISSN：1334-2606
出版年度：2006
卷号：44
期号：1
页码：59-64
语种：English
出版社：Sveuciliste u Zagrebu - Faculty of Food
摘要：The influence of carbon sources, nitrogen sources, and the addition of sucrose ester on fructanohydrolase production was investigated. The enzyme production varied depending on the carbon source. Apart from that, it was found that the enzyme activities formed by Aspergillus niger SL-09 were enhanced dramatically by the addition of sucrose ester S-770 to the medium. The effect of sucrose ester on enzyme production was also studied on molecular level, and it was confirmed that the transcription was activated by the addition of sucrose ester to the medium. The response surface methodology (RSM) was used to optimize the composition for the production of fructanohydrolase, and the enzyme activities were enhanced more than 7-fold than those obtained in the basal medium.
关键词：Aspergillus niger; fructanohydrolase; sucrose ester; optimization