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标题：VYUŽITIE ESTERÁZ AKO BIELKOVINOVÝCH MARKEROV NA DIFERENCIÁCIU GENOTYPOV JAČMEŇADIFFERENTIATION OF BARLEY GENOTYPES USING ESTERASES AS PROTEIN MARKERS
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作者：Marián Tomka ; Jana Bradová ; Zdenka Gálová 等
期刊名称：Potravinarstvo : Scientific Journal for Food Industry
印刷版ISSN：1338-0230
电子版ISSN：1337-0960
出版年度：2010
卷号：4
页码：516-522
出版社：Association HACCP Consulting
摘要：The aim of this work was to differentiate 30 barley genotypes using esterases as protein markers. We observed isoenzyme polymorphism at 4 loci (Est 1, Est 2, Est 4, Est 5). It revealed significant polymorphism using polyacrylamide gel electrophoresis. Forty three lines and 12 esterase profiles in 30 analyzed varieties were observed. A total of 13 alleles have been detected at 4 loci of which 4 alleles of Est 1 with frequencies of 2.3 – 62.8 %, 3 alleles at locus Est 2 with frequencies ranging from 4.7 to 93.0 % and 2 alleles of Est 4 with frequencies 32.6 and 67.4 %. Frequency of 4 detected alleles at locus Est 5 varied from 2.3 to 85.0 %. Ten of the 30 examined genotypes showed intravarietal polymorphism. The number of detected lines in analyzed varieties varied from 1 to 4. Two of the 43 lines were heterozygotes.
关键词：barley; esterase; polymorphism; genetic diversity; protein marker俌]?笆>笆>