文章基本信息

标题：The Three-Dimensional Structure of a Phosphorylcholine-Binding Mouse Immunoglobulin Fab and the Nature of the Antigen Binding Site
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作者：David M. Segal ; Eduardo A. Padlan ; Gerson H. Cohen 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：11
页码：4298-4302
DOI：10.1073/pnas.71.11.4298
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The structure of the Fab of McPC 603, a mouse myeloma protein with phosphorylcholine binding activity, has been determined to 3.1-A resoltuion. The four domains are found to be structurally similar with a well-defined double-layer structure. A large cavity exists at one end of the fragment, the walls of which are formed exclusively of hypervariable residues. Phosphorylcholine binds in this cavity and forms specific interactions with several well-defined amino-acid side chains of the protein. The hapten is bound asymmetrically and interacts more with the heavy chain than with the light chain.
关键词：x-ray diffraction ; domain structure ; hypervariable cavity ; hapten binding ; antibody diversity