文章基本信息

标题：Interactions of a Photo-Affinity ATP Analog with Cation-Stimulated Adenosine Triphosphatases of Human Red Cell Membranes
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作者：Boyd E. Haley ; Joseph F. Hoffman
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：9
页码：3367-3371
DOI：10.1073/pnas.71.9.3367
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：To identify and isolate ATP binding and hydrolyzing sites of human red cell membranes we have synthesized a photo-activated ATP analog, 8-azido adenosine triphosphate (N3ATP). In the absence of ultraviolet light it is a substrate for both the Mg-ATPase and the ouabain-sensitive, Na,K-ATPase. Hydrolysis of N3ATP is prevented by increasing concentrations of ATP. Photolysis of N3ATP with red cell membranes results in covalent incorporation and irreversible inhibition of both ATPase activities. Also, only three protein components of the red cell membranes are labeled. This labeling is completely abolished by appropriate concentrations of ATP.
关键词：Na,K-ATPase ; red cell ghosts ; membrane probe