文章基本信息

标题：Antibodies to Yeast Phenylalanine Transfer Ribonucleic Acid Are Specific for the Odd Nucleoside Y in the Anticodon Loop
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作者：Sara Fuchs ; Aharon Aharonov ; Michael Sela 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：7
页码：2800-2802
DOI：10.1073/pnas.71.7.2800
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Antibodies with specificity to a single species of tRNA were elicited in a goat by immunization with a glutaraldehyde conjugate of yeast phenylalanine transfer RNA with bovine gamma globulin. The specificity of the antibodies was studied by a radioimmunoassay measuring the direct binding of [3H]tRNAPhe or the inhibition of the binding. The antibodies formed are predominantly directed towards the characteristic highly modified nucleoside Y, which is located right next to the anticodon. The antibodies bind specifically to tRNAPhe, to oligonucleotides derived by enzymatic digestion from the anticodon loop of tRNAPhe, and to the Y nucleoside itself. tRNA species which do not contain Y in their sequences, or tRNAPhe from which the Y base has been excised, do not bind to the antibodies. Yeast tRNAPhe can be separated from other tRNA species with an immunoadsorbent of antibodies to tRNAPhe.
关键词：Y base ; tRNA fractionation