文章基本信息

标题：Crystals of Phosphorylcholine-Binding Fab-Fragments from Mouse Myeloma Proteins: Preparation and X-Ray Analysis
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作者：Stuart Rudikoff ; Michael Potter ; David M. Segal 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1972
卷号：69
期号：12
页码：3689-3692
DOI：10.1073/pnas.69.12.3689
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Fab-fragments of several phosphorylcholine-binding mouse-myeloma proteins have been prepared by pepsin digestion; two of these, MOPC 167 and McPC 603, gave large crystals from ammonium sulfate solutions. The Fab-fragment from MOPC 167 crystallizes in a hexagonal space group, but does not diffract to a resolution greater than about 8 A. In contrast, McPC 603 crystals (space group P63) diffract to about 2.7 A. An isotopic double-labeling technique was developed that demonstrated that the 603 crystals bind 1 mol of hapten per mol of Fab-fragment, but with a binding constant significantly lower than that observed in solution. The findings indicate that a three-dimensional model of this homogeneous antigen-binding immunoglobulin can be constructed. Accordingly, a search for heavy-atom derivatives and determination of the primary structure are in progress.
关键词：IgA ; pepsin fragments ; hapten binding ; x-ray crystallography