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  • 标题:Citrate Lyase: A Pantothenate-Containing Enzyme
  • 本地全文:下载
  • 作者:Paul A. Srere ; Brigitte Böttger ; George C. Brooks
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1972
  • 卷号:69
  • 期号:5
  • 页码:1201-1202
  • DOI:10.1073/pnas.69.5.1201
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Citrate lyase from Klebsiella aerogenes inactivated by reaction in the presence of substrate or by treatment with hydroxylamine can be reactivated with acetic anhydride only if its sulfhydryl groups are reduced. Alkaline hydrolysis of pure citrate lyase yields about 3 mol of phosphopantothenate per mol of enzyme.
  • 关键词:acetyl enzyme ; K. aerogenes ; enzyme mechanism ; hydroxylamine
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