文章基本信息

标题：Production of Altered Cell Foci by 3-Methylcholanthrene in Mouse Cells Infected with AKR Leukemia Virus
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作者：Johng S. Rhim ; Betty Creasy ; Robert J. Huebner 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：9
页码：2212-2216
DOI：10.1073/pnas.68.9.2212
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：This report describes altered cell foci observed 9-14 days after treatment with 3-methylcholanthrene of mouse-embryo tissue cultures that had previously been infected with wild-type AKR (RNA tumor) virus. The foci consisted of randomly oriented, piled-up, spindle-shaped cells. When heavily stained with Giemsa, the colonies of transformed cells were grossly visible and countable. Under the same experimental conditions, such changes of morphology were not detectable in the uninfected cells treated with 3-methylcholanthrene or in untreated cells infected with virus. The procedure may provide a rapid, quantitative test system for measurement of the oncogenic potential of certain carcinogens. As in similar previous studies in rat and hamster cells, the results suggest that the infectious, but nontransforming, RNA tumor viruses provided nascent oncogenic information, which when activated by 3-methylcholanthrene served as the specific genetic determinants of transformation.
关键词：RNA tumor viruses ; carcinogen ; benzo(a)pyrene