文章基本信息

标题：DNA Polymerase Activities and Nucleic Acid Components of Virions Isolated from a Spontaneous Mammary Carcinoma from a Rhesus Monkey
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作者：J. Schlom ; S. Spiegelman
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1971
卷号：68
期号：7
页码：1613-1617
DOI：10.1073/pnas.68.7.1613
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Virions isolated from a spontaneous mammary carcinoma of a rhesus monkey and propagated in human cells possess an RNA-instructed DNA polymerase. They also exhibit DNA polymerase activities that respond to either double-stranded DNA or synthetic RNA{middle dot}DNA hybrid complexes as templates. The virion has been shown to have a density of 1.16 g/ml and to contain a nucleic acid species of high molecular weight (sedimentation coefficient, 60-70 S), which bands as RNA at 1.670 in a Cs2SO4 equilibrium density gradient. In addition, the virions contain species of low molecular weight (4-6 S) that consist of RNA as well as components banding at densities characteristic of DNA{middle dot}RNA complexes. The nucleoid of this virion has been isolated and shown to have a density of 1.23 g/ml; it also contains a 60-70S nucleic acid species.
关键词：oncogenic RNA virus ; primate cancer