文章基本信息

标题：A mitochondrial protein associated with cytoplasmic male sterility in the T cytoplasm of maize
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作者：R. E. Dewey ; D. H. Timothy ; C. S. Levings 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1987
卷号：84
期号：15
页码：5374-5378
DOI：10.1073/pnas.84.15.5374
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Mitochondrial DNA in the T male-sterile cytoplasm (cms-T) of maize contains an open reading frame (ORF 13) associated with the T type of sterility. Antibodies raised to a chemically synthesized oligopeptide corresponding to ORF 13 were used to establish the expression of a 13-kDa protein from this reading frame. The 13-kDa polypeptide is synthesized uniquely in cms-T maize and purifies with the membrane fraction of T mitochondria. We assign the symbol urf13-T to designate this mitochondrial gene. Presence of the nuclear restorer gene Rf1 in cms-T plants results in a decrease in abundance of 13-kDa protein and alteration in the transcripts of urf13-T.
关键词：synthetic oligopeptide ; immunoprecipitation ; protein blotting ; nuclear restorer genes