文章基本信息

标题：Mosquitocidal Activity of Bti Producing Cry Protein Against Aedes aegypti Mosquito
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作者：Kazi Asraful Alam ; Shakil Ahmed Khan ; Kajla Seheli 等
期刊名称：Research Journal of Environmental Sciences
印刷版ISSN：1819-3412
电子版ISSN：2152-8238
出版年度：2008
卷号：2
期号：1
页码：46-51
DOI：10.3923/rjes.2008.46.51
出版社：Academic Journals Inc., USA
摘要：Expression and identification of putative Cry proteins of the entomopathogenic bacterium Bacillus thuringiensis israelensis (Bti) have been documented during 24, 48, 72 and 96 h of bacterial culture both at 30 and 37Â°C. Putative Cry11A and CytA, Cry4B and Cry4A have been recorded to be early, mid and late expressing proteins, respectively. No difference on the level of expression of Cry proteins has been observed between 30 and 37Â°C. The mosquitocidal proteins were found to be very thermo labile and crude protein s are effective against Aedes aegypti larvae than partial purification. These studies showed that both Cry4B and Cry4A are very unstable even at 40Â°C and therefore synergistic interactions among the Cry proteins with that of CytA which is required for high toxicity is probably hampered. These factors probably responsible for the total loss of mosquitocidal efficacy of the heat-treated partially purified proteins of Bti. Heat-treated proteins were found to loose the mosquitocidal efficacy against the larvae of Aedes aegypti mosquito.