文章基本信息

标题：An Improved System for Selection of Forward Mutations in an Escherichia coli supF Gene Carried by Plasmids.
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作者：FUMIKO OBATA ; TATSUO NUNOSHIBA ; TAMOTSU HASHIMOTO-GOTOH 等
期刊名称：Journal of Radiation Research
印刷版ISSN：0449-3060
电子版ISSN：1349-9157
出版年度：1998
卷号：39
期号：4
页码：263-270
DOI：10.1269/jrr.39.263
摘要：An improved system to examine forward mutations that occurred in the supF gene of Escherichai coli carried on a multicopy plasmid is described. The system was validated by measuring spontaneous mutations of supF plasmids propagated in wild-type, recA - and mutM - mutY - E. coli strains, the mutation frequencies of which were 1.3 × 10 -7 , 6.3 × 10 -7 and 1.5 × 10 -6 , respectively. Sequence analysis of the supF mutant plasmids revealed that G:C→T:A and G:C→C:G transversions dominated. This improved system allows rapid scoring and sequencing forward mutations in the supF gene, thus permitting its use as a genetic target for repair and mutagenesis studies in bacteria and mammalian cells.
关键词：Escherichia coli;supF gene;rpsL gene;gyrA gene;Spontaneous mutation;Transversion