文章基本信息

标题：Portable Microfluidic Integrated Plasmonic Platform for Pathogen Detection
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作者：Onur Tokel ; Umit Hakan Yildiz ; Fatih Inci 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep09152
语种：English
出版社：Springer Nature
摘要：Timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods, such as enzyme linked immunosorbent assay (ELISA), culturing or polymerase chain reaction (PCR) require long assay times, and complex and expensive instruments, which are not adaptable to point-of-care (POC) needs at resource-constrained as well as primary care settings. Therefore, there is an unmet need to develop simple, rapid, and accurate methods for detection of pathogens at the POC. Here, we present a portable, multiplex, inexpensive microfluidic-integrated surface plasmon resonance (SPR) platform that detects and quantifies bacteria, i.e. , Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) rapidly. The platform presented reliable capture and detection of E. coli at concentrations ranging from ~10⁵ to 3.2 × 10⁷ CFUs/mL in phosphate buffered saline (PBS) and peritoneal dialysis (PD) fluid. The multiplexing and specificity capability of the platform was also tested with S. aureus samples. The presented platform technology could potentially be applicable to capture and detect other pathogens at the POC and primary care settings.