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标题：AN ELECTROPHYIOLOGICAL INVESTINGATION OF TARGET SITE INSESITIVITY IN PERMETHRN-RESISTANT AND SUSCEPTIBLE STRAIN OF ANOPHELES STEPHENSI
其他标题：AN ELECTROPHYIOLOGICAL INVESTINGATION OF TARGET SITE INSESITIVITY IN PERMETHRN-RESISTANT AND SUSCEPTIBLE STRAIN OF ANOPHELES STEPHENSI
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作者：H. Vatandoost ; A. R. McCaffery ; H. Townson 等
期刊名称：Iranian Journal of Public Health
印刷版ISSN：2251-6085
电子版ISSN：2251-6093
出版年度：1998
卷号：27
期号：3-4
页码：29-44
语种：English
出版社：Tehran University of Medical Sciences
摘要：It was shown that permethrin resistance in adult Anopheles stephensi is due to at least three separate mechanisms. Cytochrome P-450s and esterases are responsible for two-thirds of the 8-fold enhanced tolerance of the DUB-APR resistant strain as compared with a sensitive strain, IND-S. It was postulated that target site insensitivity is also involved. To test this hypothesis, we conducted a neurophysiological study with the thoracic nerves of adult female An.stephensi under perfusion with saline. Spontaneous neural activity was recorded with saline alone and in the presence of permethrin. There was no change in the frequency of spontaneous firing dose of 10-3Molar perethrin rapidly produced a 3-fold increase in firing rate in the susceptible strain. In contrast, the resistant strain did not respond to this concentration. In a cumulative dose assay, the susceptible strain responded to the lowest concentration of permethrin (10-3 Molar), but the resistant strain required a much higher dose (10-8- 10+-7 molar) to produce the same rise in frequency of actin potentials. This strongly suggests that nerve insensitivity contributes to permethrin resistance in this mosquito.
关键词：An.stephensi; Target site insensitivity; Permethrin