文章基本信息

标题：Ciliary membrane vesicles of paramecium contain the voltage-sensitive calcium channel
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作者：J Thiele ; J E Schultz
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：6
页码：3688-3691
DOI：10.1073/pnas.78.6.3688
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Calcium influx into ciliary membrane vesicles from paramecia was measured by using arsenazo III as metallochromic indicator and as intravesicular calcium trap. Influx was rapid and reversible. In ciliary membrane vesicles from paramecium pawn mutants which lack the voltage-sensitive calcium channel, calcium influx was significantly less than in wild-type paramecia. These data demonstrate that the voltage-dependent calcium channel of paramecia is functional in a cell-free system and available for biochemical studies.