文章基本信息

标题：Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27
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作者：I Kumagai ; K Watanabe ; T Oshima 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：4
页码：1922-1926
DOI：10.1073/pnas.77.4.1922
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The contents of 2'-O-methylguanosine and 1-methyladenosine in unfractionated tRNA obtained from Thermus thermophilus HB27 were found to increase significantly when the bacterium was grown at a higher temperature (80 degrees C). S-Adenosyl-L-methionine-dependent tRNA (guanosine-2')-methyltransferase (EC 2.1.1.34 ) and tRNA (adenine-1)-methyltransferase (EC 2.1.1.36 ) were detected in a cell-free extract of the thermophile, and both of them were partially purified. tRNA (guanosine-2')-methyltransferase specifically catalyzed the methylation of the guanylate residue at position 19 from the 5' end of Escherichia coli tRNAMetf. The amounts of these methyltransferases in the cells and their thermal characteristics seemed to be independent of the growth temperature of the bacterial cells from which the enzymes were extracted. It was inferred that the temperature dependence of the methylation process in vivo is accounted for, not by temperature dependence of enzyme formation, but by that of the enzyme activity.