文章基本信息

标题：Efficient production of mesencephalic dopamine neurons by Lmx1a expression in embryonic stem cells
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作者：Stina Friling ; Elisabet Andersson ; Lachlan H. Thompson 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2009
卷号：106
期号：18
页码：7613-7618
DOI：10.1073/pnas.0902396106
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Signaling factors involved in CNS development have been used to control the differentiation of embryonic stem cells (ESCs) into mesencephalic dopamine (mesDA) neurons, but tend to generate a limited yield of desired cell type. Here we show that forced expression of Lmx1a, a transcription factor functioning as a determinant of mesDA neurons during embryogenesis, effectively can promote the generation of mesDA neurons from mouse and human ESCs. Under permissive culture conditions, 75%-95% of mouse ESC-derived neurons express molecular and physiological properties characteristic of bona fide mesDA neurons. Similar to primary mesDA neurons, these cells integrate and innervate the striatum of 6-hydroxy dopamine lesioned neonatal rats. Thus, the enriched generation of functional mesDA neurons by forced expression of Lmx1a may be of future importance in cell replacement therapy of Parkinson disease.
关键词：intrinsic determinant ; magnetic bead sorting ; neuroscience ; Parkinson disease ; transplantation