文章基本信息

标题：Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae
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作者：Adam M. Deutschbauer ; Roy M. Williams ; Angela M. Chu 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2002
卷号：99
期号：24
页码：15530-15535
DOI：10.1073/pnas.202604399
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have quantitatively monitored the sporulation and germination efficiencies of {approx}4,200 yeast deletion strains in parallel by using a molecular bar coding strategy. In a single study, we doubled the number of genes functionally implicated in sporulation to {approx}400, identifying both positive and negative regulators. Our set of 261 sporulation-deficient genes illustrates the importance of autophagy, carbon utilization, and transcriptional machinery during sporulation. These general cellular factors are more likely to exhibit fitness defects when deleted and less likely to be transcriptionally regulated than sporulation-specific genes. Our postgermination screening assay identified recombination/chromosome segregation genes, aneuploid strains, and possible germination-specific factors. Finally, our results facilitate a genome-wide comparison of expression pattern and mutant phenotype for a developmental process and suggest that 16% of genes differentially expressed during sporulation confer altered efficiency of spore production or defective postgermination growth when disrupted.