文章基本信息

标题：T lineage differentiation from human embryonic stem cells
本地全文：下载
作者：Zoran Galić ; Scott G. Kitchen ; Amelia Kacena 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2006
卷号：103
期号：31
页码：11742-11747
DOI：10.1073/pnas.0604244103
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Harnessing the ability of genetically manipulated human embryonic stem cells (hESC) to differentiate into appropriate lineages could revolutionize medical practice. These cells have the theoretical potential to develop into all mature cell types; however, the actual ability to develop into all hematopoietic lineages has not been demonstrated. Using sequential in vitro coculture on murine bone marrow stromal cells, and engraftment into human thymic tissues in immunodeficient mice, we demonstrate that hESC can differentiate through the T lymphoid lineage. Stable transgene expression was maintained at high levels throughout differentiation, suggesting that genetically manipulated hESC hold potential to treat several T cell disorders.
关键词：SCID-hu mouse ; T cell development ; gene therapy ; immune reconstitution ; hematopoiesis