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标题：The Antibacterial Activity of Compounds Isolated from Oakmoss against Legionella pneumophila and Other Legionella spp.
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作者：Harue Nomura ; Yasunori Isshiki ; Keisuke Sakuda 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2012
卷号：35
期号：9
页码：1560-1567
DOI：10.1248/bpb.b12-00452
出版社：The Pharmaceutical Society of Japan
摘要：Oakmoss is a natural fragrance ingredient exhibiting highly specific, potent antibacterial activity against Legionella pneumophila , a causative agent of severe water-bone pneumonia. In the present study, the antibacterial activity of individual compounds isolated from oakmoss was investigated against L. pneumophila and other Legionella spp. A total of 18 known compounds and two minor novel compounds ( i.e. , 3-methoxy-5-methylphenyl-2,4-dihydroxy-6-methylbenzoate (compound 9 ) and 8-(2,4-dihydroxy-6-(2-oxoheptyl)-phenoxy)-6-hydroxy-3-pentyl-1 H -isochromen-1-one (compound 20 )) were purified from oakmoss. The minimum inhibitory concentrations (MICs) against clinical and environmental isolates of L. pneumophila , L. bozemanii , L. micdadei , L. longbeachae , and L. dumoffii for 11 of the 20 compounds were less than 100 µg/mL (range 0.8–64.0 µg/mL). Novel compounds 9 and 20 exhibited potent antibacterial activity against L. pneumophila strains (MIC ranges of 1.3–8.0 µg/mL and 3.3–13.3 µg/mL, respectively) and also against four other Legionella species (MIC ranges of 0.8–8.0 µg/mL and 3.3–21.3 µg/mL, respectively). Time–kill assays indicated that compounds 9 and 20 kill bacteria at a concentration equivalent to 2×MIC after 1 h and 6 h co-incubations, respectively. While oakmoss and the purified components exhibited antibacterial activity against Legionella spp., they were not active against other Gram-negative and -positive bacteria such as Escherichia coli , Pseudomonas aeruginosa , Bacillus subtilis and Staphylococcus aureus .
关键词：oakmoss;antibacterial activity;Legionella pneumophila;Legionella spp.