文章基本信息

标题：Characterization and identification of glyceryl ether diesters present in tumor cells
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作者：Randall Wood ; Fred Snyder
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1967
卷号：8
期号：5
页码：494-500
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The previously unidentified neutral lipid present in tumor tissues has been isolated from Ehrlich ascites cells and unequivocally identified as a lipid class of glyceryl ether diesters containing various degrees of unsaturation, and ranging in approximate molecular weight from 760 to 990. The glyceryl ether diester fraction was shown to be free from neutral plasmalogens (glyceryl diacyl alk-1′-enyl ethers). The tumor lipid was subjected to saponification, transesterification, and lithium aluminum hydride reduction. The glyceryl monoethers that resulted from deacylation were the 1-isomers ranging in hydrocarbon chain length from C₁₂ to C₂₄. The predominant glyceryl ethers were the hexadecyl (49%), octadecyl (21%), and octadecenyl (14%) derivatives. Saturated and mono- and polyunsaturated fatty acids ranging in chain length from C₁₂ to C₂₄ carbon atoms were esterified to the glyceryl monoether. Gas-liquid chromatography, thin-layer chromatography, and nuclear magnetic resonance and infrared spectroscopy were used to characterize and identify the intact tumor lipid and its derived products.
关键词：glyceryl ether diesters ; glyceryl monoethers ; tumor lipids ; gas-liquid chromatography ; IR spectroscopy ; nuclear magnetic resonance ; thin-layer chromatography