文章基本信息

标题：Isolation analysis of tissue fatty acids by ultramicro-ozonolysis in conjunction with thin-layer chromatography and gas-liquid chromatography
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作者：O. S. Privett ; M. L. Blank ; O. Romanus 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1963
卷号：4
期号：3
页码：260-265
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Apparatus and technique for the detailed analysis of natural mixtures of fatty acids by a combination of thin-layer chromatography (TLC), gas-liquid chromatography (GLC), and reductive ozonolysis are described. The fatty acids, as methyl esters, are separated on the basis of differences in unsaturation and chain length by the application of TLC using chromatoplates coated with silver nitrate-silica gel followed by GLC. Each group of fatty acid esters is analyzed by reductive ozonolysis by an ultramicromethod. The general procedure is demonstrated with pure methyl esters and mixtures thereof, and it is applied to the analysis of the fatty acids of the liver phospholipids of rats fed a fat-free diet. The detection of endogenous 4,7,10,13-eicosatetraenoic acid of the palmitoleic acid family is reported.