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标题：Effect of a coffee lipid (cafestol) on cholesterol metabolism in human skin fibroblasts
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作者：Bente Halvorsen ; Trine Ranheim ; Marit S. Nenseter 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1998
卷号：39
期号：4
页码：901-912
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Consumption of boiled coffee promotes an elevation of plasma cholesterol concentration in humans. The active compounds found in the lipid fraction of the coffee have been identified as the diterpenes cafestol and kahweol. We have studied the effects of pure cafestol on cholesterol metabolism in human skin fibroblasts (HSF). The uptake of [¹²⁵I]-labeled tyramine cellobiose-labeled low density lipoprotein ([¹²⁵I]TC-LDL) was decreased by about 50% ( P P B _max) as well as by immunoblotting (25%) after cafestol incubation. No significant effect was observed on the level of mRNA for the LDL receptor after 11 and 23 h incubation with cafestol. Furthermore, we transfected HSF cells with a promoter region for the LDL receptor gene linked to a reporter gene, chloramphenicol acetyl transferase (CAT). No change was seen in the CAT activity after incubation with cafestol (20 μg/ml). Moreover, cafestol caused a 2.3-fold ( P 14C]oleic acid into cholesteryl esters after 24 h incubation, as compared to control cells, suggesting an increased acyl-CoA:cholesterol acyl transferase (ACAT) activity. Incorporation of [¹⁴C]acetate into cholesterol was reduced by approximately 40% ( P —Halvorsen, B., T. Ranheim, M. S. Nenseter, A. C. Huggett, and C. A. Drevon. Effect of a coffee lipid (cafestol) on cholesterol metabolism in human skin fibroblasts. J. Lipid Res. 1998. 39: 901–912.
关键词：cafestol ; 25-hydroxycholesterol ; kahweol ; low density lipoprotein ; LDL receptor