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  • 标题:Apolipoprotein H: a two-step isolation method.
  • 本地全文:下载
  • 作者:R Gambino ; G Ruiu ; M Cassader
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1996
  • 卷号:37
  • 期号:4
  • 页码:902-904
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:A new method for the purification of apolipoprotein H by affinity chromatography followed by continuous-elution electrophoresis is described. It is both simpler and less complicated than the chromatographic and electrophoretic methods usually used. In addition, apolipoprotein H is isolated in a pure, structurally uncleaved form. This is of importance, as impairment has been detected in commercial preparations. The separation and purification of apolipoprotein H is a necessary prelude to its quantitative determination and phenotyping, and hence the clarification of its physiopathological mechanisms in lipid metabolism.
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