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标题：Uptake and metabolism of triglycerides by the rat liver
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作者：Y. Stein ; B. Shapiro
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1960
卷号：1
期号：4
页码：326-331
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Triglycerides were prepared with C^l4 in the glycerol moiety and tritium in the fatty acid part as well as with over-all tritium labeling. Tracer amounts of these triglycerides were incorporated into blood plasma lipoproteins and the solutions were injected intravenously into rats. Large amounts of the radioactive material were recovered in the liver 15 minutes after the injection and the triglycerides were located predominantly in the liver mitochondria and microsomes, with relatively little in the fat droplets. Five minutes after the injection of doubly labeled triglycerides, the triglycerides recovered in the liver had practically the same H³- to C¹⁴-ratio as the injected material, indicating that intravenous lipolysis is not necessary for the uptake of neutral fat by the liver. After longer intervals the H³- to C¹⁴-ratio in the triglycerides increased rapidly, indicating intrahepatic lipolysis with the loss of free glycerol and re-esterification of the fatty acid with endogenous unlabeled glycerol precursors. Part of the tritiated fatty acids was also recovered in the phospholipid fraction, while very little of the C¹⁴-glycerol was used in phospholipid synthesis.