文章基本信息

标题：PRODUCTION of AMYLASE by A NOVEL Bacillus sp. ZBP10 in SUBMERGED FERMENTATION
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作者：Ayşe Avcı ; Dzhanser Adem ; İsmail İnan 等
期刊名称：Gida : Journal of Food
印刷版ISSN：1300-3070
电子版ISSN：1309-6273
出版年度：2016
卷号：41
期号：3
页码：131-136
DOI：10.15237/gida.GD16014
出版社：Association of Food Technology, Turkey
摘要：Bacillus sp. ZBP10 is an amylase producing strain isolated from a soil sample collected from a potato cultivation field in Sakarya. In this study, culture conditions were optimized for Bacillus sp. ZBP10 in order to increase amylase production using submerged fermentation. The effects of temperature (30-40°C), fermentation time (24-72 h), initial medium pH (6.0-9.0), carbon sources (soluble, wheat, rice and corn starches) and substrate concentration (5-30 g/L) on the production of amylase were determined. According to the results, the bacterium produced maximum amount of amylase, when the initial pH of the medium was 7.0, at 33°C, and within 48 h. Soluble starch was the best substrate among the starches tested. Optimum substrate concentration was 20 g/L for enzyme production with which 3.57±0.19 U/mL enzymatic activity was obtained.
关键词：Amylase; Bacillus; enzyme production; starch.