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标题：Farklı yumuşak astar materyallerinin Candida albicans biyofilm formasyonu açısından değerlendirilmesi
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作者：Duygu Karakış ; Canan Akay ; Demet Erdönmez 等
期刊名称：Acta Odontologica Turcica
印刷版ISSN：2147-690X
出版年度：2015
卷号：32
期号：1
页码：19-25
DOI：10.17214/aot.50404
语种：Turkish
出版社：Gazi Universitesi * Faculty of Dentistry
摘要：OBJECTIVE: The purpose of this study was to evaluate Candida albicans biofilm formation on soft liners prepared against two surfaces with different properties. MATERIALS AND METHOD: Four soft liners (Molloplast B, Permafleks, Elite-soft, Ufi Gel P) were prepared against glass or dental plaster surfaces (5x5x2 mm; n=5). The surface roughness and contact angle of the soft liner specimens were measured. Candida albicans ATCC 10231 was grown in Sabouraud dextrose broth (SDB) at 37 °C for 24 h. For biofilm formation, the specimens were incubated in polystrene wells together with fresh SDB and the C. albicans culture at 37 °C for 48 h. The specimens were stained with 0.1% crystal violet and dehydrated at 65 °C. The quantification of the C. albicans biofilm formation was performed spectrophotometrically. Comparison of the surface roughness, contact angle and biofilm formation between the materials was done using one way ANOVA and Tukey tests. Correlation between surface roughness, contact angle and biofilm formation on the specimens prepared against glass or plaster surfaces was performed using Pearson correlation analysis. RESULTS: Specimens prepared against plaster showed significantly higher surface roughness, contact angle and biofilm formation values in comparison to those prepared against glass surface (p<0.05). Among the specimens prepared against the dental plaster surface, the highest and the lowest biofilm formation values were observed in the Ufi Gel P and Molloplast B specimens, respectively. Among the specimens prepared against the glass surface, the greatest biofilm formation was shown for Ufi Gel P. There was no correlation between the surface properties (surface roughness and contact angle) and the amount of biofilm formation for the soft liner materials tested in this study (p>0.05). CONCLUSION: Although the soft liners tested in this study had similar surface topographic properties, they showed different amounts of C. albicans biofilm formation. Smooth surfaces were found to reduce the biofilm formation.
关键词：Biofilm;Candida albicans;Polydimethylsiloxanes;Biyofilm;Kandida albikans;Polidimetilsiloksanlar