文章基本信息

标题：Ethylene is involved in Trichoderma-induced resistance of bean plants against Pseudomonas syringae
本地全文：下载
作者：Agnese Gailīte ; Ineta Samsone ; Gederts Ievinsh 等
期刊名称：Environmental and Experimental Biology
印刷版ISSN：1691-8088
出版年度：2005
页码：59-70
出版社：University of Latvia
摘要：Trichodermastrains are e. ective biocontrol agents against a wide range of fungal plant pathogens. Current fi ndings suggest that, in addition to microbial antagonism, the benefi cial e. ect of Trichodermais related to activation of plant defense responses. Th e aim of the present investigation was to study the e. ect of Trichoderma harzianumon oxidative enzyme activities and resistance to Pseudomonas syringaepv. phaseolicolaof bean (Phaseolus vulgarisL.) plants. Special attention was focused on the role of ethylene as a signal mediating Trichoderma-induced responses. Both active and denatured fungal preparations induced sharp and transient increase of ethylene formation in bean leaves. Th e activity of both peroxidase (EC 1.11.1.7) and polyphenol oxidase (EC 1.10.3.2) increased in bean leaves aft er the treatment of derooted plants with active or inactivated Trichodermaformulations. Treatment with Trichodermaresulted in blocking of disease symptoms arising from inoculation with P. syringae. To demonstrate if the changes in defense responses and resistance against Pseudomonaswere ethylene-dependent, bean seedlings were pretreated with 1-methylcyclopropene, a competitive inhibitor of ethylene action. Pretreatment with 1-methylcyclopropene led to a drastic acceleration of disease development both in control and Trichoderma-treated plants. Th e activities of peroxidase and polyphenol oxidase were suppressed by 1-methylcyclopropene treatment, suggesting that ethylene acts as one of the signals in Trichoderma-enhanced resistance against P. syringae
关键词：ethylene; induced resistance; peroxidase; Phaseolus; polyphenol oxidase; Pseudomonas; Trichoderma