文章基本信息

标题：DNA DAMAGE ANALYSIS IN MAMMALIAN CELLS BY SMA-DMSO COMPLEX USING COMET ASSAY
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作者：Pallavi Singh ; Chaiti Ganguly ; F.W.Bansode 等
期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：1
页码：8734
出版社：S&S Publications
摘要：SMA-DMSO complex (RISUG) is a potent anti fertility compound developed by Prof.S.K Guha at IITKharagpur.It has been fabricated as a male contraceptive which works by lowering the pH of vas deference in males andhence damaging the sperms.Cytotoxicity of SMA-DMSO was assessed using COMET assay whereby concentration ofSMA-DMSO ranging from .001 M to 1 M was tested for inducing any significant DNA damage in Mammalian (ChineseHamster Ovary) cell line. 50 cells were scored for each concentration, with 25 cells scored from each of two replicate blindcoded slides. Statistical significance of the results was assessed by means of one-tailed Student’s t-test. Analysis of taillength and % DNA amount after treating the CHO cells with varying concentration of SMA-DMSO ,ranging from 0.001m to 1 m has shown no significant DNA damage and cytotoxicity. Results have shown RISUG to be absolutely safe forfuther usage in Mammalian cells.
关键词：SMA-DMSO; COMET Assay; CHO cell line;Cytotoxicity;Olive tail