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标题：COMPETITIVE PROTEIN?BINDING ASSAY?BASED ENZYME?IMMUNOASSAY METHOD, COMPARED TO HIGH?PRESSURE LIQUID CHROMATOGRAPHY, HAS A VERY LOWER DIAGNOSTIC VALUE TO DETECT VITAMIN D DEFICIENCY IN 9-12 YEARS CHILDREN
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作者：ZAHEDI RAD MALIHEH ; REZA NEYESTANI TIRANG ; NIKOOYEH BAHAREH 等
期刊名称：INTERNATIONAL JOURNAL OF PREVENTIVE MEDICINE
印刷版ISSN：2008-7802
出版年度：2015
卷号：6
期号：7
页码：0-0
语种：English
出版社：ISFAHAN UNIVERSITY OF MEDICAL SCIENCES
摘要：Background: The most reliable indicator of Vitamin D status is circulating concentration of 25 ? hydroxycalciferol (25 (OH) D) routinely determined by enzyme ? immunoassays (EIA) methods. This study was performed to compare commonly used competitive protein ? binding assays (CPBA) ? based EIA with the gold standard, high ? pressure liquid chromatography (HPLC).
Methods: Concentrations of 25 (OH) D in sera from 257 randomly selected school children aged 9-11 years were determined by two methods of CPBA and HPLC.
Results: Mean 25 (OH) D concentration was 22 ± 18.8 and 21.9±15.6 nmol/L by CPBA and HPLC, respectively. However, mean 25 (OH) D concentrations of the two methods became different after excluding undetectable samples (25.1±18.9 vs. 29±14.5 nmol/L, respectively; P=0.04). Based on predefined Vitamin D deficiency as 25 (OH) D<12.5 nmol/L, CPBA sensitivity and specificity were 44.2% and 60.6%, respectively, compared to HPLC. In receiver operating characteristic curve analysis, the best cut ? offs for CPBA was 5.8 nmol/L, which gave 82% sensitivity, but specificity was 17%.
Conclusions: Though CPBA may be used as a screening tool, more reliable methods are needed for diagnostic purposes.
关键词：COMPETITIVE PROTEIN-BINDING ASSAYS; HIGH-PRESSURE LIQUID CHROMATOGRAPHY; VITAMIN D DEFICIENCY; VITAMIN D MEASUREMENT