Brittle fracture properties after crack extension for structural steels under large scale cyclic loading is discussed. Fracture tests are conducted with a shallow-notched 4-point bend specimen for two structural steels, which are largely differ in work hardening properties. Loading history applied are monotonic, repetitive and incremental cyclic loading. Test results are evaluated with a 90% skeleton CTOD, δ 90% skeleton , which was defined in Part I of this study. It is shown that the 90% skeleton CTOD, is applicable to evaluate the ductile crack growth resistance curve, irrespective of loading history and work hardening properties. However, a further investigation is necessary to use the 90% skeleton CTOD as a brittle fracture controlling parameter preceded by crack growth in cyclic loading. A discussion should be focused on the effect of cyclic prestrain near the crack-tip on the embrittlement of notched components.