This study examined the physiological benefits of 24-style Taijiquan (24TJQ) exercises by comparing heart rate (HR), respiratory rate (RR), exercise intensity, electroencephalograph, surface electromyography and surface thermograph, as well as the results of physical fitness test in 20 middle-aged women (10 skilled participants and 10 novices). The data from the skilled participants showed greater values in sit-ups (p<0.01), side step (p<0.01) and stand trunk flexion (p<0.05), moreover, the statistic data demonstrated not only greater HR (p<0.05) or lower RR during exercise, but also higher beta%-power during the experiment, higher alpha%-power in the eye-closed period and central alpha dominant after exercise. These results suggest that 24TJQ is effective to promote physiological benefits in middle-aged women. It attracts strong interest and is helpful to induce psychological relaxation and mental concentration.