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标题：Wake respirometry allows breath-by-breath assessment of ventilation and CO 2 production in unrestrained animals
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作者：Kayleigh A.R. Rose ; Rory P. Wilson ; Claudia Ramenda 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：9
页码：1-15
DOI：10.1016/j.isci.2022.104878
语种：English
出版社：Elsevier
摘要：SummaryQuantifying stress and energetic responses in animals are major challenges, as existing methods lack temporal resolution and elevate animal stress. We propose “wake respirometry,” a new method of quantifying fine-scale changes in CO2production in unrestrained animals, using a nondispersive infrared CO2sensor positioned downwind of the animal, i.e., in its wake. We parameterize the dispersion of CO2in wakes using known CO2flow rates and wind speeds. Tests with three bird species in a wind tunnel demonstrated that the system can resolve breath-by-breath changes in CO2concentration, with clear exhalation signatures increasing in period and integral with body size. Changes in physiological state were detectable following handling, flight, and exposure to a perceived threat. We discuss the potential of wake respirometry to quantify stress and respiratory patterns in wild animals and provide suggestions for estimating behavior-specific metabolic rates via full integration of CO2production across the wake.Graphical abstractDisplay OmittedHighlights•We use open-path nondispersive infrared spectroscopy CO2sensor technology•We measure ventilation rate and CO2production in the wake of unrestrained animals•Rapid responses to stressors and recovery from exercise can be measured•Metabolic rate could be calculated by full integration of the wake of exhaled CO2Wildlife behavior; Physiology; Animal physiology; Methodology in biological sciences