文章基本信息

标题：A Polymerase Chain Reaction Based DNA Fingerprinting Technique: Amplified Fragment Length Polymorphism for Molecular Typing of Perchlorate Reducing Bacteria
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作者：Anita Shete 1* , Vikram Ghole 2 , Manju Raina 3 , Bikash Aich
期刊名称：Journal of Environmental Protection
印刷版ISSN：2152-2197
电子版ISSN：2152-2219
出版年度：2014
卷号：05
期号：12
页码：1111-1115
DOI：10.4236/jep.2014.512109
语种：English
出版社：Scientific Research Publishing
摘要：Genetic profiling of environmentally important organisms is very essential for easy identification of biodegrading bacteria. In the previous study, we have reported the perchlorate biodegrading bacteria and characterized them by biochemical analysis and 16 S sequencing. We have observed a very similar isolates of Arthrobacter (Actinobacteria) degrading 4.1 mM and 4.7 mM of ammonium perchlorate [1] 08D0C9EA79F9BACE118C8200AA004BA90B02000000080000000E0000005F005200650066003300390038003100390037003300340037000000 . In this study, we report PCR based DNA fingerprinting technique to generate the genomic signature of these closely related group of Arthrobacter species. This study also effectively generates unique genomic signature for each of these isolates that has potential for use in molecular monitoring as well as for tracking genomic variation and rearrangements.
关键词：AFLP—Amplified Fragment Length Polymorphism; Arthrobacter; Ammonium Perchlorate; Bio-Degradation