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标题：Berberine Inhibits Hepatic Stellate Cell Proliferation and Prevents Experimental Liver Fibrosis
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作者：Xu Sun ; Xiaodong Zhang ; Hui Hu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：9
页码：1533-1537
DOI：10.1248/bpb.32.1533
出版社：The Pharmaceutical Society of Japan
摘要：Proliferation of hepatic stellate cells (HSCs) is central for the development of fibrosis during liver injury. Our aim in this study was to determine whether berberine could inhibit HSC proliferation in vitro and prevent experimental liver fibrosis in vivo . Activated rat hepatic stellate cells (CFSCs) were incubated with various concentrations (0—20 μg/ml) of berberine. After 48 h incubation, berberine significantly inhibited CFSC proliferation and induced cell cycle arrest in G1 phase. Real-time and Western blotting revealed that both p21 and p27 expression was markedly reduced by berberine. Berberine also decreased Akt phosphorylation and FoxO1 phosphorylation, which led to FoxO1 nuclear translocation. Berberine effectively prevented CCl₄-induced liver fibrosis in mice, which was accompanied by a decrease in the number of activated HSCs. Thus, berberine was able to prevent liver fibrosis by inhibition of hepatic stellate cell proliferation.
关键词：liver fibrosis;hepatic stellate cell;berberine;cell cycle;cell proliferation