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标题：Establishment of chromium detecting kit and its application in sea water and seafood
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作者：Cuijuan Shi ; Xiujun Gao ; Qiuju Xie 等
期刊名称：E3S Web of Conferences
印刷版ISSN：2267-1242
电子版ISSN：2267-1242
出版年度：2021
卷号：252
页码：1-4
DOI：10.1051/e3sconf/202125203069
语种：English
出版社：EDP Sciences
摘要：Chromium is one of main pollution in sea water, and the establishment of detecting kit is the research hot topic. In this study, immunized mice were obtained by immunogen Cr3+-EDTA-BSA, and hybridoma strains that secrete Cr3+-EDTA monoclonal antibody were selected. Cr3+-EDTA mAb were prepared with in vivo method. Then, Cr-kit for measurement of chromium was developed, and its application was executed in sea water and seafood. Two hybridoma strains of A321 and A424 were screened. The Cr-kit standard curve of the linear range was 1.0—512 μg/L, and limit of detection was 1.0 μg/L. The average recovery rates Cr spiked in samples of sea water, clam and shrimp were 102.62%, 94.69% and 96.29%, which were accordance with the results of ICP-AES. Totally, Cr-kit was successfully developed and behaved broad application potential in sea water and seafood.