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标题：EFFECTS OF BEAUVERIA BASSIANA ISOLATES ON SITOPHILUS ORYZAE L. UNDER IN VITRO CONDITIONS
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作者：YusufYanar ; Durdane Yanar ; Dogan Sahin Budak 等
期刊名称：Fresenius Environmental Bulletin
印刷版ISSN：1018-4619
出版年度：2020
卷号：29
期号：5
页码：3422-3427
语种：English
出版社：PSP Publishing
摘要：Stored grain insect pests can reduce weight, quality, seed viability and commercial value of stored grains. Management of these pests mainly rely on synthetic insecticides and fumigants. Negative effects of these pesticides have urged the researchers to develop alternative and environmentally friendly management strategies such as microbial control. Microbial control strategy uses living organisms called microbial control agents and entomopatho- genic fungi are one of these agents. The objective of the current study was to assess the efficacy of ento- mopathogenic Beauveria bassiana isolates (F-52, F- 53 and F-56) against rice weevil (Sitophilus oryzae) and the damage they cause to stored wheat grains. Morphological analysis of the isolated fungi and molecular identification was determined using PCR technique. The efficacy of fungal isolates against S. oryzae was tested by wheat grain dipping method. Wheat grains were dipped into different conidial concertation (lxlO3, lxl05, lxlO7, lxl08, lxlO9 co- nidia/ml) for l hour. The seeds were then dried and transferred to glass vial containing ten adult insects. The vials were incubated at 25±l 0C temperature and 75% relative humidity for ll days. Thereafter, the treated adult insects were checked for mortality after lst, 3rd, 5th, 7th, and 9th days of incubation. Dead insects were transferred to moist sterile 90 mm glass Petri dishes to determine the mycosis rate. The cumulative mortality in adults 9 days after exposure to isolates of B. bassiana ranged between 22% and 94%, 3l% and l00%, and l4% and 86% for different concentrations of F-52, F-53, and F-56, respectively. The highest mortality rate (l00%) was obtained after 9 days incubation period with F-53 isolate at lxl08 conidia/ml concentration. The highest mycosis rate (95%) was obtained at the end of the l4th day with F- 52 isolate in lxl09 conidia/ml, which was followed by F-53 (93%) and F-56 (82%) isolates. The results indicated that these isolates caused high mortality and exhibited higher mycosis rate. Further storage trial should be conducted, particularly with these isolates to see their in vivo performances.
关键词：Entomopathogen;fungi;biological control;storage